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cd3 cd14 cd16 cd19  (Miltenyi Biotec)


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    Miltenyi Biotec cd3 cd14 cd16 cd19
    Cd3 Cd14 Cd16 Cd19, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd3 cd14 cd16 cd19/product/Miltenyi Biotec
    Average 94 stars, based on 28 article reviews
    cd3 cd14 cd16 cd19 - by Bioz Stars, 2026-02
    94/100 stars

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    Image Search Results


    Flow cytometric analysis of human basophil activation by salivary total proteins of silenced tissues, lipids, and protein fractions of tick Amblyomma Americanum (Am. Americanum) saliva and salivary glands. Basophils from a healthy, non-allergic control subject, depleted of IgE, were overnight primed with plasma from an individual with α-gal syndrome (α-gal sIgE = 53.7 IU/ml, total IgE = 74.8 IU/ml). These sensitized cells were then exposed to various stimuli for 30 minutes, including (A) RPMI media, (B) crosslinking anti-IgE antibody (used as a positive control), (C) protein fraction left after extraction of lipid from partially blood feed Am. americanum . tick salivary gland, (D) lipid isolated from partially blood-feed Am americanum tick salivary glands, (E) protein fraction left after extraction of lipid from saliva, (F) lipid isolated from partially blood-feed Am americanum . tick saliva,. Flow cytometry was utilized to assess CD63 expression on lineage-1 - HLA-DR - CD123 + CD203c + basophils. Results are representative of three biological replicates.

    Journal: bioRxiv

    Article Title: Identification of Alpha-Gal conjugated Lipids in Saliva of Lone-Star Tick ( Amblyomma americanum )

    doi: 10.1101/2024.02.22.581476

    Figure Lengend Snippet: Flow cytometric analysis of human basophil activation by salivary total proteins of silenced tissues, lipids, and protein fractions of tick Amblyomma Americanum (Am. Americanum) saliva and salivary glands. Basophils from a healthy, non-allergic control subject, depleted of IgE, were overnight primed with plasma from an individual with α-gal syndrome (α-gal sIgE = 53.7 IU/ml, total IgE = 74.8 IU/ml). These sensitized cells were then exposed to various stimuli for 30 minutes, including (A) RPMI media, (B) crosslinking anti-IgE antibody (used as a positive control), (C) protein fraction left after extraction of lipid from partially blood feed Am. americanum . tick salivary gland, (D) lipid isolated from partially blood-feed Am americanum tick salivary glands, (E) protein fraction left after extraction of lipid from saliva, (F) lipid isolated from partially blood-feed Am americanum . tick saliva,. Flow cytometry was utilized to assess CD63 expression on lineage-1 - HLA-DR - CD123 + CD203c + basophils. Results are representative of three biological replicates.

    Article Snippet: Stimulation reactions were halted with 20 mM EDTA, and PBMCs were stained with fluorescently labeled antibodies targeting CD123 (from BioLegend in San Diego, CA, USA), human lineage 1 markers (CD3, CD14, CD16, CD19, CD20, CD56, from BD Biosciences in San Jose, CA, USA), HLA-DR, CD63 (from eBiosciences ThermoFisher in Waltham, MA, USA), and CD203c (from IOTest Beckman Coulter in Marseille, France).

    Techniques: Activation Assay, Positive Control, Extraction, Isolation, Flow Cytometry, Expressing